Nano-CT Scanner and Staining Technique Allow for High-Resolution 3D Histology

Frank
May 14, 2018
13 Views

Researchers at the Technical University of Munich have developed a nano-CT scanning technique. When coupled with a new staining technique, their nano-CT scanner can take extremely high-resolution scans of intact tissue samples, such as tumors. The new method allows clinicians to examine soft tissue samples without the need for sectioning or toxic stains.

At present, examining soft tissue samples frequently involves sectioning them and mounting them on slides. This is time-consuming and laborious and makes it difficult to envisage the intact structure of the tissue. Imaging intact pieces of tissue to generate a 3D reconstruction could save time and help clinicians to analyze such samples.

One option is CT scanning, but current micro- or nano-CT scanners frequently lack the resolution to effectively scan soft tissue samples for clinical applications. In addition, soft tissue is particularly difficult to image using CT, requiring toxic stains which are often time-consuming to apply, and can significantly affect the tissue, making it impossible to analyze it further.

The Munich research team has previously developed a high-resolution nano-CT scanner that can achieve resolutions of 100 nanometers, by using a newly developed x-ray source and a highly focused x-ray beam. Recently, the researchers developed a staining system that allows their nano-CT scanner to effectively scan tissue samples for histological investigations.

By pretreating the tissue samples, the researchers were able to use eosin, a standard histological stain, and achieve high-resolution tissue scans. “Our approach included developing a special pre-treatment so that we can use eosin anyway,” said Madleen Busse, a researcher involved in the study. “Another important benefit is that there are no problems using established methods to examine the tissue sample following the scan.”

The research team was able to scan large tissue segments, such as an intact mouse kidney, and the scans included similar levels of information as tissue sections. However, at present, the team envisages that this type of CT histology will be useful as a supplementary technique, rather than replacing conventional methods. “Alongside diagnostic applications, the non-destructive 3D examination enabled by Nano-CT could deliver new insights into the microscopic origins of widespread diseases such as cancer,” said Franz Pfeiffer, another researcher involved in the study.

Here’s a video demonstrating the imaging resolution achieved using the new nano-CT technique:

Flashback: CT Scanning at Resolutions Down to 100nm Unveiled by German Researchers…

Study in PNAS: Three-dimensional virtual histology enabled through cytoplasm-specific X-ray stain for microscopic and nanoscopic computed tomography

Via: Technical University of Munich…

Frank’s source: https://www.medgadget.com/2018/02/nano-ct-scanner-staining-technique-allow-high-resolution-3d-histology.html

 

You may be interested

Overcoming the stumbling blocks of digital health adoption
About Health
0 shares0 views
About Health
0 shares0 views

Overcoming the stumbling blocks of digital health adoption

Frank - May 23, 2018

Achieving perfection in the realm of digital health adoption is far from an easy feat. Numerous barriers prevent perfect deployment,…

Jean-Claude Van Damme’s youngest son arrested after ‘holding his flatmate at knifepoint’
About Entertainment
0 shares2 views
About Entertainment
0 shares2 views

Jean-Claude Van Damme’s youngest son arrested after ‘holding his flatmate at knifepoint’

Frank - May 23, 2018

Hollywood star Jean-Claude Van Damme's youngest son has been arrested for allegedly taking his flatmate prisoner with a knife. Police were…

Star Wars 9: Release date for Episode IX has been delayed
About Entertainment
0 shares2 views
About Entertainment
0 shares2 views

Star Wars 9: Release date for Episode IX has been delayed

Frank - May 23, 2018

After Star Wars: Episode IX's dramatic change in personnel, which saw J.J. Abrams step in as director after Colin Trevorrow's…

Leave a Comment

Your email address will not be published.

8 + nineteen =